![]() Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice, and discard the pellet.leukocytes need a very light centrifugation). You may have to vary the centrifugation force and time depending on the cell type a guideline is 20 min at 12,000 rpm but this must be determined for your experiment (e.g. Centrifuge in a microcentrifuge at 4☌.Maintain constant agitation for 30 min at 4☌.Alternatively cells can be trypsinized and washed with PBS prior to resuspension in lysis buffer in a microcentrifuge tube. Scrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microcentrifuge tube. ![]()
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